Recombinant TMF1 antibody

Catalog No. ABIN7882429

The Rabbit Monoclonal anti-TMF1 antibody is suitable to detect TMF1 in samples from Human. It has been validated for WB, ICC and IF.

Quick Overview for Recombinant TMF1 antibody (ABIN7882429)

Target: TMF1 (TATA Element Modulatory Factor 1 (TMF1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TMF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 30T88

Product Details anti-TMF1 Antibody

Purpose: TMF1 Antibody / TATA element modulatory factor

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human TMF was used as the immunogen for the TMF1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TMF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the TMF1 antibody at -20oC.

Target Details for TMF1

Target: TMF1 (TATA Element Modulatory Factor 1 (TMF1))

Alternative Name: TMF1

Background: TMF1 antibody recognizes TATA element modulatory factor, a nuclear protein encoded by the TMF1 gene. TATA element modulatory factor was first identified for its ability to bind DNA elements and regulate transcription, particularly through interactions with transcription initiation complexes. TMF1 functions as a cofactor influencing promoter activity and has roles in transcriptional repression, Golgi organization, and vesicular trafficking. Studies with TMF1 antibody have shown that this protein can act as both a positive and negative regulator depending on context, allowing cells to fine tune the initiation of gene expression. Its broad functional spectrum has made it a focus in transcriptional regulation, intracellular transport, and cancer biology.

At the molecular level, TMF1 interacts with transcription factors such as the TATA binding protein and modulates their activity, thereby influencing the expression of a variety of genes. This modulation can occur through direct protein-protein interactions or by altering chromatin structure to make promoter regions more or less accessible. Beyond transcriptional regulation, TMF1 is associated with the Golgi apparatus, where it helps maintain organelle structure and mediates transport of vesicles. Its involvement in Golgi positioning and vesicle budding has highlighted TMF1 as a dual-function protein, bridging gene regulation and organelle dynamics. Studies employing TMF1 antibody have demonstrated its importance in proper protein sorting and secretion, adding another layer to its regulatory portfolio.

Dysregulation of TATA element modulatory factor has been implicated in cancer and developmental disorders. Altered TMF1 expression can influence cell proliferation and differentiation, linking this protein to oncogenic pathways. Its role in spermatogenesis is of particular note, with evidence showing that reduced expression of TMF1 impairs spermatid development, leading to infertility in experimental models. In cancer research, both upregulation and downregulation of TMF1 have been reported, suggesting context-dependent roles in tumor progression. Detection with TMF1 antibody enables exploration of these diverse functions, and recent studies have suggested possible connections between TMF1 expression and clinical outcomes in certain malignancies.

TMF1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting reveals protein levels across tissues and cell lines, while immunohistochemistry highlights nuclear localization patterns that reflect its transcriptional regulatory roles. Immunofluorescence can further resolve subcellular localization within the Golgi network and nucleus, helping to define the multifunctional nature of this protein. In some experimental settings, TMF1 antibody has been used in chromatin immunoprecipitation to identify DNA binding partners and confirm direct gene regulatory roles. Researchers studying gene regulation, intracellular transport, fertility, and disease mechanisms rely on TMF1 antibody to generate reproducible and interpretable results.

By supporting experiments across molecular biology, cancer research, and developmental biology, NSJ Bioreagents provides validated TMF1 antibody reagents that facilitate discovery of the complex functions of TATA element modulatory factor. As interest grows in proteins that integrate transcriptional control with intracellular transport, TMF1 antibody offers a critical tool for advancing understanding of these dual processes.

UniProt: P82094

Pathways: Hormone Transport