Thyroglobulin antibody

Catalog No. ABIN7882471

The Mouse Monoclonal anti-Thyroglobulin antibody is suitable to detect Thyroglobulin in samples from Human. It has been validated for IHC (p).

Quick Overview for Thyroglobulin antibody (ABIN7882471)

Target: Thyroglobulin (TG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thyroglobulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM221

Product Details anti-Thyroglobulin Antibody

Purpose: Anti-Thyroglobulin Antibody

Purification: Protein G affinity chromatography

Immunogen: Human thyroid follicular cells were used as the immunogen for this anti-Thyroglobulin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The optimal dilution of the anti-Thyroglobulin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Thyroglobulin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Alternative Name: Thyroglobulin

Background: Thyroglobulin is a 660 kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.

UniProt: P01266

Pathways: Thyroid Hormone Synthesis