Recombinant TIMM22 antibody

Catalog No. ABIN7882539

This Rabbit Monoclonal antibody specifically detects TIMM22 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant TIMM22 antibody (ABIN7882539)

Target: TIMM22 (Translocase of Inner Mitochondrial Membrane 22 Homolog (TIMM22))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TIMM22 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 31T40

Product Details anti-TIMM22 Antibody

Purpose: TIMM22 Antibody / Translocase of inner mitochondrial membrane 22

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human TIMM22 was used as the immunogen for the TIMM22 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TIMM22 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the TIMM22 antibody at -20oC.

Target Details for TIMM22

Target: TIMM22 (Translocase of Inner Mitochondrial Membrane 22 Homolog (TIMM22))

Alternative Name: TIMM22

Background: TIMM22 antibody detects translocase of inner mitochondrial membrane 22, a mitochondrial import protein encoded by the TIMM22 gene. TIMM22 is part of the TIM22 complex, which mediates insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. It recognizes hydrophobic precursor proteins delivered by the small TIM chaperone complex and inserts them into the inner membrane in an energy-dependent process. Through this activity, TIMM22 ensures proper biogenesis of mitochondrial carriers and maintenance of energy metabolism.

TIMM22 antibody is widely applied in mitochondrial biology and protein trafficking research. By detecting TIMM22, scientists can study how mitochondrial import machinery regulates oxidative phosphorylation and cellular energy production. Defects in TIMM22 function lead to impaired assembly of carrier proteins such as ADP/ATP translocases, disrupting mitochondrial function and cellular homeostasis.

Western blot assays detect TIMM22 protein in mitochondrial fractions, while immunohistochemistry highlights expression in tissues with high metabolic demand such as muscle, heart, and brain. Immunofluorescence reveals punctate mitochondrial staining, consistent with its localization in the inner membrane. These methods allow precise analysis of mitochondrial import processes.

Mutations in TIMM22 and disruption of mitochondrial protein import are linked to metabolic disorders, neurodegeneration, and cardiomyopathy. By applying TIMM22 antibody, researchers can investigate how defects in import machinery contribute to disease and explore therapeutic strategies to restore mitochondrial function.

TIMM22 antibody from NSJ Bioreagents provides dependable specificity for studying mitochondrial protein import and energy metabolism. Its strong performance across applications ensures accurate detection of TIMM22 in diverse biological systems.

UniProt: Q9Y584