TFF1 antibody

Catalog No. ABIN7882562

The Mouse Monoclonal anti-TFF1 antibody (Clone TFF1-2133) (ABIN7882562) specifically detects TFF1 in IF, IHC (p) and FACS. The antibody is reactive with Human samples.

Quick Overview for TFF1 antibody (ABIN7882562)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFF1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Grade: Carrier-free

Clone: TFF1-2133

Product Details anti-TFF1 Antibody

Purpose: pS2 Antibody / TFF1 (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for this pS2 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The optimal dilution of the pS2 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the pS2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: pS2

Background: It recognizes a polypeptide of 6.5 kDa, identified as pS2 estrogen-regulated protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60 % of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

UniProt: P04155

Pathways: EGFR Signaling Pathway