Recombinant UFD1L antibody

Catalog No. ABIN7882625

The Rabbit Monoclonal anti-UFD1L antibody (Clone 29U95) (ABIN7882625) specifically detects UFD1L in WB, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant UFD1L antibody (ABIN7882625)

Target: UFD1L (Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This UFD1L antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 29U95

Product Details anti-UFD1L Antibody

Purpose: UFD1L Antibody / Ubiquitin Fusion Degradation 1 Like

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human UFD1L was used as the immunogen for the UFD1L antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the UFD1L antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the UFD1L antibody at -20oC.

Target Details for UFD1L

Target: UFD1L (Ubiquitin Fusion Degradation Protein 1 Homolog (UFD1L))

Alternative Name: UFD1L

Background: UFD1L antibody detects Ubiquitin fusion degradation protein 1 like, encoded by the UFD1L gene. Ubiquitin fusion degradation protein 1 like forms a complex with NPL4 and the ATPase p97/VCP to regulate extraction of ubiquitinated substrates from membranes and protein complexes. This activity is essential for protein quality control, endoplasmic reticulum associated degradation, and mitotic progression. UFD1L antibody provides researchers with an important tool for studying ubiquitin signaling, proteostasis, and cell cycle regulation.

The UFD1L-NPL4-p97 complex functions as a segregase, using ATP hydrolysis to unfold and extract ubiquitinated proteins for degradation by the proteasome. Research using UFD1L antibody has demonstrated that this complex acts in multiple pathways, including clearance of misfolded proteins from the endoplasmic reticulum, disassembly of protein aggregates, and regulation of spindle assembly during mitosis. Its versatile roles make UFD1L a central hub in protein quality control networks.

Mutations and deletions involving UFD1L are associated with genetic disorders. Studies with UFD1L antibody have shown that haploinsufficiency contributes to DiGeorge syndrome, a developmental disorder characterized by congenital heart defects, immune dysfunction, and craniofacial anomalies. Because UFD1L is located in the 22q11 region, deletions that remove this gene disrupt proteostasis during development, contributing to disease phenotypes.

In cancer biology, UFD1L has been implicated in regulating cell proliferation and survival. Research using UFD1L antibody has revealed that overexpression enhances proteasomal degradation of regulatory proteins, supporting uncontrolled growth. Conversely, loss of function can cause accumulation of toxic aggregates, impairing viability. These dual roles highlight its importance in maintaining balanced proteostasis across different contexts.

UFD1L antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting identifies endogenous levels, immunohistochemistry localizes expression in developmental tissues, and immunoprecipitation demonstrates interactions with p97/VCP and NPL4. These applications make UFD1L antibody essential in both developmental and disease research.

By supplying validated UFD1L antibody reagents, NSJ Bioreagents supports studies into proteostasis, ER associated degradation, and developmental biology. Detection of Ubiquitin fusion degradation protein 1 like provides insight into how protein quality control influences health and disease.

UniProt: Q92890