Recombinant USP5 antibody

Catalog No. ABIN7882627

The Rabbit Monoclonal anti-USP5 antibody is suitable to detect USP5 in samples from Human, Mouse and Rat. It has been validated for WB, IF and ICC.

Quick Overview for Recombinant USP5 antibody (ABIN7882627)

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This USP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 32U18

Product Details anti-USP5 Antibody

Purpose: USP5 Antibody / Ubiquitin specific peptidase 5

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human USP5 was used as the immunogen for the USP5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the USP5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the USP5 antibody at -20oC.

Target Details for USP5

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Alternative Name: USP5

Background: USP5 antibody detects ubiquitin specific peptidase 5, a deubiquitinating enzyme encoded by the USP5 gene. USP5 is part of the ubiquitin specific protease family that cleaves ubiquitin from polyubiquitin chains. Its primary function is to disassemble unanchored polyubiquitin, recycling ubiquitin monomers for reuse in the ubiquitin proteasome system. Through this activity, USP5 maintains ubiquitin homeostasis, regulates protein turnover, and influences signaling pathways that depend on ubiquitination.

USP5 antibody is commonly used in research on protein degradation, signal transduction, and cancer biology. By detecting USP5, scientists can study how ubiquitin processing enzymes control proteostasis and prevent accumulation of abnormal proteins. Dysregulation of USP5 activity leads to disruptions in protein quality control and contributes to disease.

In western blot assays, USP5 antibody detects protein bands corresponding to the enzyme in cell and tissue extracts. Immunohistochemistry reveals localization in cytoplasmic and nuclear compartments, while immunofluorescence highlights its presence in proteasome rich regions. These applications make USP5 antibody versatile for tracking ubiquitin protease activity.

USP5 has been implicated in cancer progression, where it regulates stability of tumor suppressors and oncogenes. Elevated USP5 expression supports tumor cell survival and proliferation by maintaining ubiquitin pools and stabilizing growth promoting proteins. By applying USP5 antibody, researchers can assess how deubiquitinating enzymes contribute to malignancy and therapy resistance.

Beyond cancer, USP5 plays roles in neurodegeneration, immune regulation, and viral infection. In neurons, altered USP5 activity contributes to proteotoxic stress and synaptic dysfunction. In immunity, it regulates signaling pathways dependent on ubiquitination, affecting cytokine responses and pathogen defense. This broad functional spectrum highlights the research value of USP5 antibody in multiple biological contexts.

USP5 antibody from NSJ Bioreagents provides reliable specificity for examining ubiquitin processing, proteostasis, and disease related pathways. Its performance across methods makes it an essential reagent for laboratories investigating the ubiquitin system.

UniProt: P45974