Recombinant CrkL antibody

Catalog No. ABIN7882648

The Rabbit Monoclonal anti-CrkL antibody is suitable to detect CrkL in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, ICC, IP and FACS.

Quick Overview for Recombinant CrkL antibody (ABIN7882648)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 32C63

Product Details anti-CrkL Antibody

Purpose: CrkL Antibody / Crk-like protein

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human CrkL was used as the immunogen for the CRKL antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CRKL antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the CRKL antibody at -20oC.

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CrkL

Background: CrkL antibody detects Crk-like protein, encoded by the CRKL gene. Other identifiers include v-crk avian sarcoma virus CT10 oncogene homolog-like, adaptor protein CRKL, and CRK-like proto-oncogene. CrkL is a member of the Crk family of adaptor proteins that contain SH2 and SH3 domains. These modular domains mediate interactions with phosphorylated tyrosines and proline-rich motifs, enabling CrkL to assemble signaling complexes downstream of receptor tyrosine kinases, cytokine receptors, and integrins. By linking upstream receptors to downstream effectors, CrkL regulates processes such as cell adhesion, migration, proliferation, and survival.

CrkL antibody is widely applied in cancer biology, immunology, and signal transduction research. CrkL is required for BCR-ABL signaling in chronic myeloid leukemia, where it mediates oncogenic transformation and survival. CrkL phosphorylation status serves as a biomarker for monitoring tyrosine kinase inhibitor therapy in leukemia patients. Beyond cancer, CrkL regulates immune receptor signaling, T cell activation, and integrin-mediated adhesion.

Applications for CrkL antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blotting detects CrkL protein and phosphorylation isoforms, immunohistochemistry maps expression in tumors and lymphoid tissues, and immunofluorescence highlights localization at focal adhesions and signaling complexes. These methods provide comprehensive tools for analyzing CrkL biology in both normal and disease contexts.

Dysregulation of CrkL contributes to leukemia, solid tumors, and immune disorders. Overexpression and abnormal phosphorylation promote cell transformation, invasion, and therapy resistance. By applying CrkL antibody, researchers can evaluate CrkL as a diagnostic marker and therapeutic target. In development, CrkL supports neural crest migration and organogenesis, linking it to congenital malformations when disrupted.

CrkL interacts with proteins including CBL, paxillin, p130CAS, and STAT5, orchestrating diverse signaling cascades. Antibody-based detection allows detailed dissection of these interactions across pathways. NSJ Bioreagents provides CrkL antibody with validated specificity, supporting reliable detection in cancer, immunology, and signaling studies.

UniProt: P46109

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor