Recombinant WDR4 antibody

Catalog No. ABIN7882696

The Rabbit Monoclonal anti-WDR4 antibody is suitable to detect WDR4 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for Recombinant WDR4 antibody (ABIN7882696)

Target: WDR4 (WD Repeat Domain 4 (WDR4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This WDR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 25W87

Product Details anti-WDR4 Antibody

Purpose: WDR4 Antibody / WD repeat domain 4

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human WDR4 was used as the immunogen for the WDR4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the WDR4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the WDR4 antibody at -20oC.

Target Details for WDR4

Target: WDR4 (WD Repeat Domain 4 (WDR4))

Alternative Name: WDR4

Background: WDR4 antibody detects WD repeat domain 4, a conserved protein involved in RNA modification and tRNA methylation. WDR4 forms a complex with METTL1 to catalyze the N7-methylguanosine modification of tRNAs, a modification required for translational fidelity and RNA stability. The WDR4 antibody is widely used in molecular biology and RNA research to study RNA methylation, protein translation, and gene expression regulation.

WDR4 is encoded by the WDR4 gene located on human chromosome 21q22.3. The protein is approximately 412 amino acids long and contains several WD40 repeats that mediate protein-protein interactions, forming a beta-propeller structure typical of the WD repeat family. WDR4 localizes to the nucleus and cytoplasm, where it partners with METTL1 to catalyze methylation of specific tRNA guanine residues, ensuring proper translation initiation and elongation.

The WDR4 antibody detects a 46 kilodalton protein by western blot and demonstrates nuclear and cytoplasmic staining patterns under immunofluorescence. Through its interaction with METTL1, WDR4 stabilizes the tRNA methyltransferase complex and regulates global protein synthesis. Loss of WDR4 leads to reduced tRNA methylation, impaired translation, and increased sensitivity to stress.

Mutations in WDR4 cause a spectrum of developmental and neurological disorders, including microcephaly and intellectual disability. These defects arise from disrupted tRNA modification, which affects the translation of growth-related and neuronal genes. In cancer, overexpression of WDR4 enhances translation of oncogenic proteins and promotes tumor progression through metabolic reprogramming.

Because of its key role in RNA modification and translation regulation, WDR4 serves as an essential model for understanding epitranscriptomic control of gene expression. NSJ Bioreagents provides a validated WDR4 antibody optimized for its applications, supporting studies of RNA methylation, translational regulation, and disease pathogenesis.

UniProt: P57081