CEA antibody

Catalog No. ABIN7884238

The Mouse Monoclonal anti-CEA antibody is suitable to detect CEA in samples from Human. It has been validated for IHC and ELISA.

Quick Overview for CEA antibody (ABIN7884238)

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Fragment: F(ab')2 fragment

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEA antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: C234

Product Details anti-CEA Antibody

Purpose: Carcinoembryonic antigen (CEA) F(ab')2 Mouse Monoclonal Antibody (C234)

Specificity: This antibody is CEA specific. It does not recognize NCA, neither in enzyme linked immunosorbent assay (ELISA) not on Western blots of crude tissue extracts. This antibody binds to CEA producing human adenocarcinoma cells (e.g. LS174T,WiDr) but does not bind to CEA non-secreting human adenocarcinoma cells (Colo 320DM). It does not immunostain cryocuts of any normal human tissues tested (artery, heart muscle, kidney, large bowel, liver, lung, skeletal muscle, skin, small gut, spleen, testes or vein) neither does it react with any antigens on human blood cells when separated by fluoresceine actuated cell sorter (FACS). Immunohistochemical analysis of cancerous tissues revealed that the antibody bound selectively adenocarcinoma and not carcinoid tumor of the colon, as well as tumor- associated CEA in breast, gastric and lung neoplasms, while there was no immunohistochemical evidence of cross-reactivity with NCA. A remarkable feature of this antibody is its ability to distinguish immunohistochemically between cancer and residual normal tissue within the same organ.

Purification: Purified

Immunogen: Physically modified CEA

Isotype: IgG kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Buffer: Lyophilized Protein A purified in PBS pH 7.4 (F(ab'2))

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Alternative Name: Carcinoembryonic antigen (CEA)

Background:

CEA

Carcinoembronic antigen (CEA), an oncodevelopmental tumor marker is a fairly typical, high-molecular weight, cell surface glycoprotein over-expressed in adenocarcinomas of entero-dermally-derived digestive system epithelia and in fetal colon. The majority of antibodies induced against CEA would cross-react with a variety of proteins present in normal tissues such as non-specific cross-reacting antigen (NCA). Highly specific anti-CEA mouse monoclonal antibody was produced as a result of a unique imunization protocol.