Chromogranin A antibody

Catalog No. ABIN7884239

The Rabbit Polyclonal anti-Chromogranin A antibody (ABIN7884239) specifically detects Chromogranin A in IF and WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for Chromogranin A antibody (ABIN7884239)

Target: Chromogranin A (CHGA)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB)

Product Details anti-Chromogranin A Antibody

Purpose: Catestatin Rabbit Polyclonal Antibody

Specificity: The antibody recognizes the human Catestatin (CHGA352-372)

Purification: Purified

Immunogen: Catestatin 21AA fragment

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: If reconstituted with deionized water in 25 μL: WB: 1:4000, IF: 1:500. Optimal dilution has to be determined by the user.

Buffer: Lyophilised purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: Catestatin

Background:

Cat7, Cst

Chromogranin-A is a secreted protein known as a ""prohormone"" which is found in endocrine cells and neurons and may be released upon stimulation. It is thought to be involved in the formation of dense core granules, where it may be processed to form the bioactive peptide serpinin, which is thought to regulate the biogenesis of dense core granules in endocrine cells. Catestatin is a 21 amino acids fragment (352 to 372) from Chromogranin A. It's a bioactive peptide that can stimulate histamine release from mast cells and it's an inhibitor of catecholamines release. Catestatin has been shown to also have cardiovascular functions and antimicrobial activity.

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling