Enkephalin, Leu antibody

Catalog No. ABIN7884241

The Mouse Monoclonal anti-Enkephalin, Leu antibody is suitable to detect Enkephalin, Leu in samples from Human and Rat. It has been validated for IHC, ICC and RIA.

Quick Overview for Enkephalin, Leu antibody (ABIN7884241)

Target: Enkephalin, Leu

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Radioimmunoassay (RIA)

Clone: NOC1

Product Details

Purpose: Leu-enkephalin Mouse Monoclonal Antibody (NOC1)

Specificity: A sensitive antibody against enkephalinergic fibrilar networks in the globus pallidus, N. accumbens, septum, hypothalamus, thalamus, amygdala, N. interstitialis of the stria terminalis and brain stem nuclei. This antibody does not distinguish between Met5-enkephalin and Leu5-enkephalin by radioimmunoassay or by immunocytochemistry. It is recommended for localizing enkephalinergic sites by immunohistochemistry. It does not bind to beta-endorphin or dynorphin-containing areas. It exhibits approximately. 40 % cross reactivity with c-terminal extended Met-enkephalin hexapeptides and 7 % cross reactivity with the extended heptapeptide (-Arg-Phe-0H), but does not recognize other endogenous peptides. All wellestablished enkephalin immunoreactive sites are recognized by this antibody in immunohistochemistry (IHC) and electron microscopy immunocytochemistry (ICC). Specific for: rat and human

Immunogen: pentapeptide ( Tyr-Gly-Gly-Phe-Leu)

Isotype: IgG1

Application Details

Application Notes: IHC 1:100

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 0.5 mL: IHC 1:100 - 1:400. Optimal dilution has to be determined by the user.

Buffer: Lyophilized tissue culture supernatant

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details

Target: Enkephalin, Leu

Alternative Name: Leu-enkephalin

Background:

Enkephalin, endorphins, endorphin, Met5-enkephalin, Leu5-enkephalin, Met-enkephalin, Leu-enkephalin, anti-Enkephalin, anti-endorphins, anti-endorphin, anti-Met5-enkephalin, anti-Leu5-enkephalin, anti-Met-enkephalin, anti-Leu-enkephalin

Enkephalins (penta-peptides) have been discovered as regulators of nerve impulses involving pain in the brain. It has been shown that these peptides act as natural analgesics (pain-killers) and their action mimics that of morphine and other opiates. At present, it is thought that the morphine-like effects are due to aromatic side chains on phenylanine and tyrosine which mimic a similar structure on morphine. Research suggests that it does not make much difference whether the enkephalin contains methionine or leucine at the acid end of the peptide for its action.