HIV-1 Transmembrane Glycoprotein (HIV-1 gp41) (AA 579-613) antibody

Catalog No. ABIN7884243

This Human Monoclonal antibody specifically detects HIV-1 Transmembrane Glycoprotein in ELISA, IP and ICo. It exhibits reactivity toward Human Immunodeficiency Virus (HIV).

Quick Overview for HIV-1 Transmembrane Glycoprotein (HIV-1 gp41) (AA 579-613) antibody (ABIN7884243)

Target: HIV-1 Transmembrane Glycoprotein (HIV-1 gp41)

Reactivity: Human Immunodeficiency Virus (HIV)

Host: Human

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Immunocompetition (ICo)

Clone: 50-69

Product Details

Binding Specificity: AA 579-613

Purpose: HIV-1 (gp41) Human Monoclonal Antibody (50-69)

Specificity: The antibody recognizes the HIV-1 protein gp41. (amino acids 579-613)

Purification: Protein G purified

Immunogen: The antibody was generated from HIV-infected individuals by a method based on the Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMCs) followed by fusion with heteromyeloma cells.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with deionized water. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details

Target: HIV-1 Transmembrane Glycoprotein (HIV-1 gp41)

Alternative Name: HIV-1 (gp41)

Target Type: Viral Protein

Background: Of the two types of Human immunodeficiency virus (HIV) which have been characterized, HIV-1 is more infective and is prevalent globally. The HIV virion is encapsulated by a viral envelope that contains complexes of surface proteins, which are important for the virus to attach and fuse to infect target cells. A precursor molecule known as gp160 (glycoprotein 160) is cleaved by proteases into gp120 (glycoprotein 120) and gp41 (glycoprotein 41), glycoproteins that make up the envelope proteins of HIV. Glycoprotein gp41 is embedded in the viral membrane and can interact with other gp41 proteins. The proteins gp41 and gp120 form a complex which is thought to undergo conformational changes in order to initiate membrane fusion of the virus with a cell.