PODXL2 antibody (AA 121-150)

Catalog No. ABIN7884248

The Rat Monoclonal anti-PODXL2 antibody (Clone F4B10) (ABIN7884248) specifically detects PODXL2 in FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for PODXL2 antibody (AA 121-150) (ABIN7884248)

Target: PODXL2 (Podocalyxin-Like 2 (PODXL2))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PODXL2 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: F4B10

Product Details anti-PODXL2 Antibody

Binding Specificity: AA 121-150

Purpose: Endoglycan Rat Monoclonal Antibody (F4B10)

Specificity: The antibody recognizes the extracellular domain of Endoglycan protein (aa121-150)

Immunogen: Peptide

Isotype: IgM

Application Details

Application Notes: FC 1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 200 μL: FC 1:400. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein free tissue culture supernatant

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for PODXL2

Target: PODXL2 (Podocalyxin-Like 2 (PODXL2))

Alternative Name: Endoglycan

Background:

Podocalyxin-like protein 2, Podxl2

Endoglycan is a CD34-related sialomucin protein highly expressed in the brain. It is also expressed on hematopoietic precursors, endothelial cells, smooth muscle cells, leukocyte subpopulations and is weakly expressed in liver. Endoglycan can act in selectin-dependant adhesion of leukocytes to vasculature and it also can be involved in inhibition of stem cell differentiation.