Substance P antibody

Catalog No. ABIN7884252

This Mouse Chimeric antibody specifically detects Substance P in IHC and ELISA. It exhibits reactivity toward Human, Crab and Pigeon.

Quick Overview for Substance P antibody (ABIN7884252)

Target: Substance P

Reactivity: Human, Crab, Pigeon

Host: Mouse, Rat

Clonality: Chimeric

Conjugate: This Substance P antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: P4C1

Product Details anti-Substance P Antibody

Purpose: Substance P / HRP Bispecific Monoclonal Antibody (P4C1)

Specificity: This is a bispecific antibody which recognizes (A) Substance P and (B) Horseradish peroxidase types I, II, VI, IX, X. This antibody recognizes the COOH-terminal end of substance P. It does not recognize Leu- or Met enkephalin, somatostatin or beta-endorphin, cross-reactivity with eledoisin: 5 % . It recognizes Substance P in post-mortem tissue of the human brain.

Predicted Reactivity: All Mammals

Immunogen: Substance P and Full Lenght HRP

Isotype: IgG1, IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 2 ml: IHC: 1:100 - 1:200. Optimal dilution has to be determined by the user.

Buffer: Lyophilized tissue culture supernatant

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for Substance P

Target: Substance P

Background:

Substance P, tachykinin, neuropeptide, neurokinin, SP, anti-tachykinin, anti-neuropeptide, anti-neurokinin, anti-SP

This antibody is a bispecific monoclonal antibody capable of simultaneously recognize the horseradish peroxidase (HRP) and Substance P. Bi-specific monoclonal antibodies have been successfully used in one step immunocytochemistry and immuno-dot assays. These results demonstrated that bispecific antibodies can be applied in quick and simple staining procedures and provide intense immunostaining. The P4C1 antibody has been successfully used in immunoassays and immunohistochemistry. Light and electron micrographs of rat tissue developed using this antibody clearly demonstrate substance P immunoreactive sites with very minimal non-specific background binding.