Aggrecan antibody (Intracellular, N-Term)

Catalog No. ABIN7884748

The Rabbit Polyclonal anti-Aggrecan antibody is suitable to detect Aggrecan in samples from Human, Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for Aggrecan antibody (Intracellular, N-Term) (ABIN7884748)

Target: Aggrecan (ACAN)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aggrecan antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-Aggrecan Antibody

Binding Specificity: AA 169-181, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to SLC25A12

Predicted Reactivity: Rat,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KDKSKSGMISGLD, corresponding to amino acid residues 169-181 of mouse SLC25A12

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237025)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Aggrecan

Target: Aggrecan (ACAN)

Alternative Name: AGC1

Background:

Synonyms: AGC1, ARALAR1, Solute carrier family 25 member 12, Mitochondrial aspartate glutamate carrier 1, Calcium-binding mitochondrial carrier protein Aralar1, Araceli hiperlarga

Description: SLC25 is a large family of nuclear-encoded transporters embedded in the inner mitochondrial membrane and in a few cases other organelle membranes.SLC2512A is a gene encoding the aspartate/glutamate carrier 1 (AGC1). AGC1 possesses six transmembrane alpha helices and N- and C-termini on the cytosolic side of the membrane1.AGC1 catalyzes an exchange between intra-mitochondrial aspartate and cytosolic glutamate, an important step in urea synthesis. As a component of the malate-aspartate shuttle (MAS), AGC1 also has a role in the transfer of NADH, carrying reducing equivalents from the cytosol into the mitochondrial matrix. AGC1 is expressed predominantly in heart and skeletal muscle, with weaker expression in brain and kidney. AGC1 is the main AGC isoform present in the adult brain, and it is expressed mainly in neurons. As part of the MAS, AGC1 has a role in the physiology of pancreatic β-cells by influencing glucose-induced activation of mitochondrial metabolism and insulin secretion and it is the only AGC isoform expressed in pancreatic islets and β-cells. AGC1 has several Ca2+-binding EF-hand motifs located on its long, hydrophilic amino-terminus which extends into the intermembrane space and its activity is strongly regulated by extra-mitochondrial Ca2+. Dysregulation of cellular Ca2+ homeostasis mediated by AGC1 has been implicated in the pathogenesis of autistic spectrum disorder2.

Gene ID: 78830

UniProt: Q8BH59

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport