Anoctamin 5 antibody (2nd Cytoplasmic Loop)

Catalog No. ABIN7884760

The Rabbit Polyclonal anti-Anoctamin 5 antibody is suitable to detect Anoctamin 5 in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for Anoctamin 5 antibody (2nd Cytoplasmic Loop) (ABIN7884760)

Target: Anoctamin 5 (ANO5)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Anoctamin 5 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-Anoctamin 5 Antibody

Binding Specificity: 2nd Cytoplasmic Loop, AA 532-544

Purpose: A Rabbit Polyclonal Antibody to Anoctamin-5 Channel

Predicted Reactivity: Rat - identical, human - 12,13 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)TKMEIPRTHQEYE, corresponding to amino acid residues 532-544 of mouse ANO5

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234743)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Anoctamin 5

Target: Anoctamin 5 (ANO5)

Alternative Name: ANO5

Background:

Synonyms: ANO5, Transmembrane protein 16E, TMEM16E, GDD1, LGMD2L

Description: Anoctamins, also known as TMEM16, is a family of ten proteins involved in a variety of functions including phospholipid scrambling, ion transport and the regulation of other membrane proteins. ANO1 (Anoctamin-1), ANO2 and ANO5 function as Ca2+-activated Cl- channels (CaCCs) and play an important role in calcium dependent chloride secretion. All members of the Anoctamin family present high structural homology and consist of eight transmembrane domains and cytosolic N- and C-termini1.The ANO5 gene is widely distributed and expressed in bone, skeletal muscle, cardiac muscle, brain, lung and kidney and also in the mucosal layer of the GI tract and the skeletal muscle layer of the esophagus of mice2.In phylogenetic analysis ANO5 was found to be a close relative of ANO6. ANO6 has an association with phospholipid scramblase activity. Under both physiological and pathological conditions scramblase dissipates the normal asymmetry of the cell membrane by catalyzing the rapid externalization of phospholipids on the cell membrane and thus promoting apoptosis3.Recessive mutations in the ANO5 gene were found to be linked to limb-girdle muscular dystrophies type 2L (LGMD2L) and non-dysferlin Miyoshi myopathy4. LGMD2L is characterized by proximal weakness, asymmetric quadriceps femoris and atrophy of the biceps brachii muscle5.Mutations in the gene are also linked to the Gnathodiaphyseal dysplasia disease, an unusual generalized autosomal dominant disease. Patients with this syndrome show sclerosis of tubular bones, lesions of jawbones, an increased sensitivity for jaw infection and osteomyeletis and fragile bones6.

Gene ID: 233246

UniProt: Q75UR0