BRS3 antibody (2nd Extracellular Loop)

Catalog No. ABIN7884790

The Rabbit Polyclonal anti-BRS3 antibody is suitable to detect BRS3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, FACS and LCI.

Quick Overview for BRS3 antibody (2nd Extracellular Loop) (ABIN7884790)

Target: BRS3 (Bombesin-Like Receptor 3 (BRS3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-BRS3 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 186-201

Purpose: A Rabbit Polyclonal Antibody to Bombesin Receptor 3

Predicted Reactivity: Rat,mouse - 13,16 amino acids residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SNVYTFRDPNKNMTFE, corresponding to amino acid residues 186-201 of human BRS3 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:500

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:50

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234864)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for BRS3

Target: BRS3 (Bombesin-Like Receptor 3 (BRS3))

Alternative Name: BB3R

Background:

Synonyms: BB3R, BRS3

Description: Bombesin receptor 3 (BB3) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was first isolated from frog skin and it was later established that mammalians express endogenous bombesin like peptides such as gastrin-releasing peptide (GRP) a 27 amino acid homologue and neuromedin B (NMB) a 10 amino acid homologue.1BB1 is the preferred receptor for NMB while BB2 is the preferred receptor for GRP.BB3 was the third member of the bombesin receptors family to be cloned based in its sequence similarity to BB1 and BB2.2 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors as is its affinity for NMB and GRP. This suggests that the mammalian endogenous ligand for the BB3 receptor remains to be identified.All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB3 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization and cell growth.3The physiological function of the BB3 receptor is not clear. However, studies with BB3 receptor deficient mice have shown that the mice develop mild obesity associated with hypertension and reduced metabolic rate.4,5Finally, BB3 receptor expression has been identified in several human tumors most notably lung. BB3 is therefore considered a potential target for the development of both diagnostics and anti-cancer therapies.6

Gene ID: 680

UniProt: P32247

Pathways: Feeding Behaviour