CHERP antibody (Cytosolic)

Catalog No. ABIN7884835

The Rabbit Polyclonal anti-CHERP antibody is suitable to detect CHERP in samples from Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for CHERP antibody (Cytosolic) (ABIN7884835)

Target: CHERP (Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHERP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-CHERP Antibody

Binding Specificity: AA 669-684, Cytosolic

Purpose: A Rabbit Polyclonal Antibody to CHERP

Predicted Reactivity: Rat,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KLEDHEYKPLDPKDIR, corresponding to amino acid residues 669-684 of mouse CHERP

Isotype: IgG

Application Details

Application Notes:

WB: 1:1500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235001)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CHERP

Target: CHERP (Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP))

Alternative Name: Calcium homeostasis endoplasmic reticulum protein

Background:

Synonyms: Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6

Description: CHERP (calcium homeostasis endoplasmic reticulum protein) is a ubiquitously expressed integral sarco/endoplasmic reticulum (SR/ER) membrane protein. CHERP protein consist of 916 amino acid in humans and its structure containing a Pro-rich region and a segment of Arg-Ser dipeptide repeats (an RS domain) near the C terminus, and an RNA recognition motifs (RRMs)1-3.CHERP acts as a regulator of both major families of endoplasmic reticulum Ca2+ channels, inositol 1,4,5-trisphosphate receptors (IP3Rs) and ryanodine receptors (RyRs). CHERP is responsible for the regulation of intracellular Ca2+ mobilization in human cells and is involved in neuroblastoma cell proliferation, apoptosis and tumorigenicity1,2.CHERP co-localizes with IP3 receptors throughout the cytoplasmic and perinuclear regions in human erythroleukemia cells and in Jurkat cells. CHERP is also detected in the nucleoplasm with prominent accumulation at nuclear speckles1,2. CHERP co-localizes with endogenous ryanodine receptor type 1 (RyR1) in the sarcoplasmic reticulum of rat soleus muscle. Studies of CHERP knockdown reveal decreased intracellular Ca2+ mobilization, cell growth and proliferation2,3.

Gene ID: 27967

UniProt: Q8CGZ0