CMKLR1 antibody (2nd Extracellular Loop)

Catalog No. ABIN7884871

The Rabbit Polyclonal anti-CMKLR1 antibody is suitable to detect CMKLR1 in samples from Mouse. It has been validated for WB and IHC.

Quick Overview for CMKLR1 antibody (2nd Extracellular Loop) (ABIN7884871)

Target: CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CMKLR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-CMKLR1 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 201-215

Purpose: A Rabbit Polyclonal Antibody to Chemerin Receptor 1

Predicted Reactivity: Rat only

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HSTHPRTDPVGYSRH, corresponding to amino acid residues 201-215 of rat Chemerin receptor 1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CMKLR1

Target: CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))

Alternative Name: Chemokine-like receptor 1

Background:

Synonyms: Chemokine-like receptor 1, Cmklr1, Chemerin1, Gpcr27, G-protein coupled receptor ChemR23, CHEMERINR, G-protein coupled chemoattractant-like receptor, G-protein coupled receptor DEZ

Description: Chemerin Receptor 1 belongs to class A G-protein coupled receptors. The receptor is coupled to Gi/o, leading to an increase in intracellular cAMP and Ca2+ following activation by the binding of Chemerin, its endogenous ligand. Like all GPCRs, it has seven transmembrane domains, an extracellular N-terminus and intracellular C-terminal tail1.Chemerin Receptor 1 has numerous names depending on how the gene encoding the receptor was cloned: Chemokine-like receptor 1, CMKLR1, ChemR23 and others all correspond to the same gene/protein1.Expression of Chemerin receptor 1 at the mRNA level is largely detected in adipose tissue, and skin dermal layer. At the protein level, CMKLR1 is expressed at the cell surface of dendritic cells, monocytes and macrophages, smooth muscle cells, adipose tissue, lung, lymph nodes and testis1.A role for the receptor in the inflammatory response has been attributed, however data indicate contradictory roles, proinflammatory and anti-inflammatory functions of the receptor. Expression of Chemerin receptor 1 in adipose tissue clearly establishes its involvement in energy metabolism as an important regulator of adipogenesis1.

Gene ID: 60669

UniProt: O35786