CRMP1 antibody (Intracellular)

Catalog No. ABIN7884890

The Rabbit Polyclonal anti-CRMP1 antibody (ABIN7884890) specifically detects CRMP1 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CRMP1 antibody (Intracellular) (ABIN7884890)

Target: CRMP1 (Collapsin Response Mediator Protein 1 (CRMP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRMP1 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-CRMP1 Antibody

Binding Specificity: AA 481-496, Intracellular

Purpose: A Rabbit Polyclonal Antibody to CRMP-1

Predicted Reactivity: Mouse - identical, human - 12,16 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RVRIRSKVFGLHSVSR, corresponding to amino acid residues 481-496 of rat CRMP1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235158)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CRMP1

Target: CRMP1 (Collapsin Response Mediator Protein 1 (CRMP1))

Alternative Name: CRMP-1

Background:

Synonyms: CRMP-1, Collapsin response mediator protein 1, Dihydropyrimidinase-related protein 1, DRP-1, Dpysl1, Dihydropyrimidinase-like 1, Ulip3, Unc-33-like phosphoprotein 3

Description: Collapsin response mediator proteins (CRMPs) are a family of cytosolic phosphoproteins that were originally identified in developing nervous tissue. They are characterized as intracellular mediators of Semaphorin 3A signaling pathway, which is involved in axon differentiation during neural development.The CRMP family includes five members, CRMP-1 to CRMP-5. All five isoforms share a significant similarity in sequence with liver dihydropyrimidinase (DHPase) and with each other. CRMP-1 to CRMP-4 share 75 % identical sequence, while CRMP-5 shares 50 % identity with the other CRMPs.CRMP-1 is involved in cortical neuronal migration through reelin signaling. CRMP-1 inhibits the migration and invasion of non-small cell lung cancer (NSCLC). CRMP-1 includes a long form isoform, LCRMP-1, which promotes filopodia formation, cancer cell migration and invasion, resulting in poor clinical outcomes in NSCLC patients, therefore the long form is considered to be an invasion enhancer in NSCLC1-3.Human CRMP-1 overall structure is very similar to mouse CRMP-1. The human protein generally comprises a heterotetrameric assembly with a randomly coiled link that connects the protomers to each other. The proteins can form homotetramers when purified from bovine brain. CRMPs contains a positively charged C-terminal region that is highly susceptible to proteolysis2,3.

Gene ID: 25415

UniProt: Q62950