DRD3 antibody (Extracellular, N-Term)

Catalog No. ABIN7884919

The Rabbit Polyclonal anti-DRD3 antibody is suitable to detect DRD3 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF, FACS and LCI.

Quick Overview for DRD3 antibody (Extracellular, N-Term) (ABIN7884919)

Target: DRD3 (Dopamine Receptor D3 (DRD3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free, KO Validated

Product Details anti-DRD3 Antibody

Binding Specificity: AA 15-29, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to D3 Dopamine Receptor

Predicted Reactivity: Mouse - identical, human - 12,15 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: CGAENSTGVNRARPH, corresponding to amino acid residues 15-29 of rat DRD3 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235243)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for DRD3

Target: DRD3 (Dopamine Receptor D3 (DRD3))

Alternative Name: DRD3

Background:

Synonyms: DRD3

Description: The D3 Dopamine Receptor (D3 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and schizophrenia.1All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1The D3 receptor distribution in the brain is relatively restricted to limbic areas such as striatum, islands of Calleja and olfactory tubercle. In the periphery, it is expressed in the kidney particularly in proximal tubules.1The exact physiological function of the D3 receptor remains to be fully elucidated. In studies using D3 receptor knockout mice, the most prominent dysfunction was the development of rennin-dependent hypertension. Potential roles in reinforcement and reward behaviors have also been suggested as well as roles in neuropsychiatric disorders such as drug abuse and schizophrenia.2

Gene ID: 29238

UniProt: P19020

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Proton Transport, Negative Regulation of Transporter Activity