CD39 antibody (Extracellular Loop)

Catalog No. ABIN7884923

The Rabbit Polyclonal anti-CD39 antibody (ABIN7884923) specifically detects CD39 in WB, FACS and LCI. The antibody is reactive with Human and Rat samples.

Quick Overview for CD39 antibody (Extracellular Loop) (ABIN7884923)

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD39 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-CD39 Antibody

Binding Specificity: AA 398-411, Extracellular Loop

Purpose: A Rabbit Polyclonal Antibody to CD39

Predicted Reactivity: 14 amino acid residues identical, rat - 11,Mouse - 12

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KTSYAGVKEKYLSE, corresponding to amino acid residues 398-411 of human CD39

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235254)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CD39

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Alternative Name: Ectonucleoside triphosphate diphosphohydrolase 1

Background:

Synonyms: Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Ecto-ATPDase 1, Ecto-apyrase, Lymphoid cell activation antigen, ENTPD1

Description: Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase1, ENTPD1, or CD39) is an enzyme that catalyzes the phosphohydrolysis of extracellular adenosine triphosphate (eATP) and diphosphate (eADP), that are released following inflammatory stress and cell injury, to adenosine monophosphate (AMP). AMP is then used by the ecto-5'-nucleotidase CD73 to synthesize adenosine1.Hydrolysis of adenosine triphosphate and adenosine diphosphate, performed by CD39, suppresses the immune system and inhibits T-cell and Natural killer (NK) cell responses. This inhibition has a role in promoting Chronic lymphocytic leukemia (CLL) and hence it is proposed that blockade or inhibition of CD39 may be a target for new immune therapy for CLL2. CD39 activity is also related to the occurrence of calcific aortic valve disease (CAVD)3, and to various other types of immune responses4. In addition, CD39 plays a dominant role in the purinergic regulation of inflammation1.The transcription of CD39 is constitutive in microvascular endothelium and certain immune cells. The modulated expression of NTPDase1 has been closely associated with inflammatory cytokines, oxidative stress and hypoxia in vitro and in vivo5.Localization of CD39 within lipid rafts suggests that this ecto-enzyme may be involved in cell-cell contact and signaling6.CD39 is a part of the ectonucleoside triphosphate dyphosphohydrolases (eNTDPase) protein family which shares a unique structural feature: instead of being anchored in the membrane by a single transmembrane domain or lipid link like other ectoenzymes, these proteins contain two transmembrane domains, one at each end of the protein. The active site lies in the large extracellular region. In addition, all family members share a set of short sequences termed apyrase conserved regions (ACRs)7.

Gene ID: 953

UniProt: P49961