SH3G2 antibody (Intracellular)

Catalog No. ABIN7884928

The Rabbit Polyclonal anti-SH3G2 antibody is suitable to detect SH3G2 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for SH3G2 antibody (Intracellular) (ABIN7884928)

Target: SH3G2 (Endophilin-A1 (SH3G2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3G2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SH3G2 Antibody

Binding Specificity: AA 255-269, Intracellular

Purpose: A rabbit polyclonal antibody to Endophilin-A1

Predicted Reactivity: Rat - identical. Human - 14 out of 15 amino acids identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)YQPKPRMSLEFATGD, corresponding to amino acid residues 255 - 269 of mouse SH3GL2

Isotype: IgG

Application Details

Application Notes: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SH3G2

Target: SH3G2 (Endophilin-A1 (SH3G2))

Alternative Name: SH3GL2

Background:

Synonyms: SH3 domain-containing GRB2-like protein 2, EEN-B1, SH3 domain protein 2A, SH3GL2

Description:

Endophilin-1 is a member of the Endophilin family, a group of proteins characterized by the presence of BAR (Bin/Amphiphysin/Rvs) and SH3 (Src Homology 3) domains.

The Endophilin family is primarily divided into two subfamilies. Endophilin A , that includes subunits A1, A2 and A3, is predominantly involved in membrane remodeling processes such clathrin-mediated and clathrin-independent endocytic pathways, especially in neurons. The Endophilin B subfamily includes subunits B1 and B2, and is more implicated in mitochondrial dynamics, autophagy and apoptosis.1,2

Endophilin-A1 is expressed almost exclusively in the brain, particularly in synaptic terminals in neurons, consistent with a neuronal specialization for membrane trafficking and synaptic function. It is essential for synaptic vesicle endocytosis, where it recruits and organizes proteins at sites of high membrane curvature to facilitate the formation and recycling of clathrin-coated vesicles at synaptic terminals. It is critical for dendritic spine morphogenesis, synaptic stabilization, and plasticity, supporting both excitatory and inhibitory synaptic organization.3,4

Changes in Endophilin-1 expression are linked to several neurodegenerative disease, most notably, Alzheimer's (AD) and Parkinson disease. Increased expression of Endophilin-1 has been observed in the brains of patients with AD and in transgenic mouse models of the disease.5,6

Endophilin-1 was identified as a Parkinson's disease risk locus and in addition, it was found to be upregulated in a mouse model of the disease.7,8

In summary, Endophilin-A1 not only serves as a compelling target for understanding synaptic biology but also holds promise as a biomarker and therapeutic candidate in neurodegenerative and neuropsychiatric diseases.

Gene ID: 20404

UniProt: Q62420

Pathways: Neurotrophin Signaling Pathway, EGFR Downregulation