GPR4 antibody (Extracellular Loop)

Catalog No. ABIN7884985

The Rabbit Polyclonal anti-GPR4 antibody is suitable to detect GPR4 in samples from Human, Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for GPR4 antibody (Extracellular Loop) (ABIN7884985)

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-GPR4 Antibody

Binding Specificity: AA 162-177, Extracellular Loop

Purpose: A Rabbit Polyclonal Antibody to GPR4

Predicted Reactivity: Rat,mouse,human - 15,16 amino acid residues identical.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235415)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR4

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Alternative Name: GPR4

Background:

Synonyms: Acid-sensing G-protein coupled receptor 4, GPR19

Description: G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.

Gene ID: 308408

UniProt: Q4KLH9