GPR65 antibody (Extracellular Loop)

Catalog No. ABIN7884991

The Rabbit Polyclonal anti-GPR65 antibody is suitable to detect GPR65 in samples from Human, Mouse and Rat. It has been validated for FACS, LCI and WB.

Quick Overview for GPR65 antibody (Extracellular Loop) (ABIN7884991)

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR65 antibody is un-conjugated

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI), Western Blotting (WB)

Grade: Carrier-free

Product Details anti-GPR65 Antibody

Binding Specificity: AA 72-84, Extracellular Loop

Purpose: A Rabbit Polyclonal Antibody to GPR68 (OGR1)

Predicted Reactivity: Rat,mouse,human - 14,15 amino acid residues identical.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235434)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR65

Target: GPR65 (G Protein-Coupled Receptor 65 (GPR65))

Alternative Name: G-protein coupled receptor 65

Background:

Synonyms: Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Description: All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus1. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54 % ) with the human receptor GPR42.GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP3) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H+-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation4.

Gene ID: 238377

UniProt: Q8BFQ3

Pathways: cAMP Metabolic Process