SLC6A1 antibody (2nd Extracellular Loop)

Catalog No. ABIN7885006

The Rabbit Polyclonal anti-SLC6A1 antibody is suitable to detect SLC6A1 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF, FACS and LCI.

Quick Overview for SLC6A1 antibody (2nd Extracellular Loop) (ABIN7885006)

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-SLC6A1 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 194-206

Purpose: A Rabbit Polyclonal Antibody to GABA Transporter 1 (GAT-1)

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GAT-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235465)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SLC6A1

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Alternative Name: GABATR

Background:

Synonyms: GABATR, GABT1, GABATHG, SLC6A1, Na+/Cl-- dependent GABA transporter 1

Description: γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3. GAT-1 activity can be regulated at the level of its plasma membrane expression which is in part regulated by its trafficking via phosphorylation of residues in intracellular loops1.GAT-1 expression is restricted to the central nervous system, specifically to axon terminals, in the neocortex and in some astrocytic processes4.GAT-1 may be implicated in various psychological and neurological disorders such as schizophrenia, epilepsy and cerebral ischemia1,5-7.

Gene ID: 79212

UniProt: P23978