HRH1 antibody (3rd Intracellular Loop)

Catalog No. ABIN7885076

The Rabbit Polyclonal anti-HRH1 antibody (ABIN7885076) specifically detects HRH1 in WB, IHC, IF, FACS and IC. The antibody is reactive with Mouse and Rat samples.

Quick Overview for HRH1 antibody (3rd Intracellular Loop) (ABIN7885076)

Target: HRH1 (Histamine Receptor H1 (HRH1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC)

Grade: Carrier-free

Product Details anti-HRH1 Antibody

Binding Specificity: 3rd Intracellular Loop

Purpose: A Rabbit Polyclonal Antibody to Histamine H1 Receptor

Predicted Reactivity: Mouse - identical, human,dog - 13,14 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat HRH1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235750)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HRH1

Target: HRH1 (Histamine Receptor H1 (HRH1))

Alternative Name: H1R

Background:

Synonyms: H1R, HH1R

Description: Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.1H1 receptors are expressed in many cell types including endothelial and smooth muscle cells where they mediate vasodilatation and increased vascular permeability effects. H1R is widely distributed through the brain where it mediates sleep-wake cycles and regulates cognitive functions. The receptor is expressed in several cell populations involved in the immune response, including T-cells and B-cells, monocytes and eosinophiles.2,3H1 anti-histamine compounds have been available for almost thirty years and are among the most commonly used medication for conditions such as allergic inflammation. In addition, H1 anti-histamines are also prescribed for the treatment of insomnia and analgesia.2,3

Gene ID: 24448

UniProt: P31390

Pathways: Regulation of Carbohydrate Metabolic Process