HRH1 antibody (Extracellular Loop)

Catalog No. ABIN7885077

This Rabbit Polyclonal antibody specifically detects HRH1 in WB, IHC, IF, FACS and LCI. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HRH1 antibody (Extracellular Loop) (ABIN7885077)

Target: HRH1 (Histamine Receptor H1 (HRH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-HRH1 Antibody

Binding Specificity: AA 176-191, Extracellular Loop

Purpose: A Rabbit Polyclonal Antibody to Histamine H1 Receptor

Predicted Reactivity: Mouse, human - 14,16 amino acid residues identical,rat - 15

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235747)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HRH1

Target: HRH1 (Histamine Receptor H1 (HRH1))

Alternative Name: Histamine Receptor H1

Background:

Synonyms: Histamine Receptor H1, H1R

Description: Histamine, acts via distinct histamine H1, H2, H3, and H4 receptors, and regulates various physiological and pathological processes. Hence the activity of Histamine receptors is very crucial. They are members of G-protein coupled receptors superfamily. Thus, have seven transmembrane domains, extracellular N-terminal tail and a cytoplasmic C-terminus1.H1 receptor is excitatory and couples to Gq-type proteins, activating a signaling cascade which eventually leads to an increased cellular concentration of Ca2+ 3.H1 receptor is implicated in type I hypersensitivity allergic reactions caused by numerous allergens1. An allergic reaction occurs when allergens activate mast cells that release histamine, the released histamine binds to H1R and this causes an activation of a cascade that leads to vasodilation and vascular hyper-permeability, resulting in allergic inflammation.H1R is expressed in various tissues throughout body, including the airway, vascular smooth muscle, and brain2. H1R expressed in the brain plays an important role in the regulation of sleep-arousal cycle and memory by binding histamine where it acts as a neurotransmitter1.

Gene ID: 15465

UniProt: P70174

Pathways: Regulation of Carbohydrate Metabolic Process