HRH3 antibody (3rd Intracellular Loop)

Catalog No. ABIN7885079

The Rabbit Polyclonal anti-HRH3 antibody (ABIN7885079) specifically detects HRH3 in WB, IHC, IF and IC. The antibody is reactive with Rat and Mouse samples.

Quick Overview for HRH3 antibody (3rd Intracellular Loop) (ABIN7885079)

Target: HRH3 (Histamine Receptor H3 (HRH3))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: Carrier-free

Product Details anti-HRH3 Antibody

Binding Specificity: 3rd Intracellular Loop

Purpose: A Rabbit Polyclonal Antibody to Histamine H3 Receptor

Predicted Reactivity: Mouse - identical, human 14,16 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RTRLRLDGGREAGPE, corresponding to amino acids 228-242 of rat HRH3 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235758)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HRH3

Target: HRH3 (Histamine Receptor H3 (HRH3))

Alternative Name: H3R

Background:

Synonyms: H3R, HH3R, GPCR97

Description: Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H3 receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, activation of the mitogen-activated protein kinase (MAPK) cascade and inhibition of the Na+/H+ exchanger.1,2H3 receptors are expressed primarily in the central nervous system (CNS) where they are located in presynaptic membranes of histaminergic neurons, where they negatively regulate the synthesis and release of histamine. In addition, H3 receptors are also located on nonhistaminergic neurons, where they regulate the release of other amines such as dopamine, serotonin, and norepinephrine.2,3Based on these studies, a central role for H3 receptors has been proposed in disorders involving cognition such as attention deficit and hyperactivity disorder (ADHD), Alzheimer disease and Schizophrenia, as well as sleep and energy homeostasis (i.e. obesity) disorders.2,3

Gene ID: 85268

UniProt: Q9QYN8

Pathways: cAMP Metabolic Process, Feeding Behaviour