HRH4 antibody (1st Extracellular Loop)

Catalog No. ABIN7885080

This Rabbit Polyclonal antibody specifically detects HRH4 in WB, FACS and LCI. It exhibits reactivity toward Human.

Quick Overview for HRH4 antibody (1st Extracellular Loop) (ABIN7885080)

Target: HRH4 (Histamine Receptor H4 (HRH4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-HRH4 Antibody

Binding Specificity: 1st Extracellular Loop

Purpose: A Rabbit Polyclonal Antibody to Human Histamine H4 Receptor

Predicted Reactivity: Won't recognize rat,mouse H4 Histamine Receptor

Purification: Affinity purified on immobilized antigen.

Immunogen: HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human HRH4

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HRH4

Target: HRH4 (Histamine Receptor H4 (HRH4))

Alternative Name: H4R

Background:

Synonyms: H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35

Description: Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1- H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.Histamine H4 receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade.1,2Histamine H4 receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H4 receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H4 receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.2,3These studies indicate that histamine H4 receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.2,3

Gene ID: 59340

UniProt: Q9H3N8