HOMER1 antibody (Intracellular)

Catalog No. ABIN7885081

The Rabbit Polyclonal anti-HOMER1 antibody is suitable to detect HOMER1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for HOMER1 antibody (Intracellular) (ABIN7885081)

Target: HOMER1 (Homer Homolog 1 (HOMER1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOMER1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-HOMER1 Antibody

Binding Specificity: AA 293-296, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Homer1

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KQEIDNARELQEQR, corresponding to amino acid residues 293-296 of rat Homer1

Isotype: IgG

Application Details

Application Notes:

WB: 1:500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235762)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HOMER1

Target: HOMER1 (Homer Homolog 1 (HOMER1))

Alternative Name: Homer protein homolog 1

Background:

Synonyms: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47

Description: Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1.The long form of Homer1 assembles group 1 metabotropic glutamate receptors mGluR1 and mGluR5 in large macromolecular complexes at synapses as well as with components of the NMDA receptor complex at the neuronal cell membrane. In contrast, the short form of Homer1 lacks the ability of linking mGluR1/5 to synaptic proteins and functions as an endogenous negative modulator of the mGluR1/5-inositol 1,4,5-trisphosphate receptor signaling complex. Transgenic mice with overexpressed Homer1 in striatal medium spiny neurons demonstrate normal development of striatal structure and afferent/efferent connectivity. However, motor performance in behavioral tasks and striatal responses to the psychomotor stimulant amphetamine are significantly altered in these mice2.At concentrations

Gene ID: 29546

UniProt: Q9Z214

Pathways: Skeletal Muscle Fiber Development