HOMER2 antibody (C-Term, Intracellular)

Catalog No. ABIN7885082

The Rabbit Polyclonal anti-HOMER2 antibody (ABIN7885082) specifically detects HOMER2 in WB, IHC and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for HOMER2 antibody (C-Term, Intracellular) (ABIN7885082)

Target: HOMER2 (Homer Homolog 2 (HOMER2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOMER2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-HOMER2 Antibody

Binding Specificity: AA 302-316, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Homer2

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EDKVRSLKTDIEESK, corresponding to amino acid residues 302-316 of rat Homer2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235766)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HOMER2

Target: HOMER2 (Homer Homolog 2 (HOMER2))

Alternative Name: Homer protein homolog 2

Background:

Synonyms: Homer protein homolog 2, Cupidin, Vesl-2

Description: Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1. The Homer family includes three members, Homer1b/c, Homer2a/b, and Homer3. Homer2 protein, also called Vesl-2, is a scaffolding protein that is important for the proper organization, localization and signaling of excitatory postsynaptic receptors. Homer2 protein associates with filamentous actin (F-actin) and directly binds to drebrin, an actin-binding and remodeling protein in dendritic spines. The protein also binds to activated Cdc42, a member of the Rho small GTPase family2. The protein interacts with mGluR1, mGluR5, PI3 Kinase enhancer (PIKE), inositol 1,4,5-trisphosphate receptors (IP3), SHANK and other proteins3,4.Deletion of the Homer2 gene in mice demonstrates several abnormalities in glutamatergic signaling and in the nucleus accumbens such as reduced basal extracellular glutamate content and reduced function and total protein expression of both mGluR1 and the cystine-glutamate exchanger4.

Gene ID: 29547

UniProt: O88801

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling