HOMER3 antibody (Intracellular)

Catalog No. ABIN7885083

This Rabbit Polyclonal antibody specifically detects HOMER3 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HOMER3 antibody (Intracellular) (ABIN7885083)

Target: HOMER3 (Homer Homolog 3 (Drosophila) (HOMER3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOMER3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-HOMER3 Antibody

Binding Specificity: AA 155-169, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Homer3

Predicted Reactivity: Human - identical, mouse - 14,15 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RSQSADAPGPTERER, corresponding to amino acid residues 155 - 169 of rat Homer3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235770)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HOMER3

Target: HOMER3 (Homer Homolog 3 (Drosophila) (HOMER3))

Alternative Name: Homer protein homolog 3

Background:

Synonyms: Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3

Description: The HOMER protein family includes Homer1, 2, and 3. These proteins are mainly expressed in the neuronal post-synaptic density (PSD) where they play a role in post-synaptic signaling and receptor trafficking by forming multivalent links with various receptors and PSD scaffolding proteins. The expression level of Homer proteins in non-neural tissues is very low in comparison with that in the nervous system, HOMER3 also expressed in the lung, spleen, kidney and ovary. In the mouse brain HOMER3 is concentrated in the dendritic spines of Purkinje cells connecting the parallel fibers and is also present in their axons, In the hippocampus, HOMER3 is concentrated in the CA2-CA3 regions1,2.All three proteins in the HOMER family have several isoforms as a result of alternative splicing, HOMER3 are shown to exist in both long and short forms, the latter without the C-terminal coiled-coil domains. HOMER3 mRNA is subject to alternative splicing at five different sites giving rise to ten variants of HOMER3 proteins. HOMER1b and HOMER3 have been shown to form a tetramer via the carboxy-terminal domain3.HOMER3 directly binds to type I metabotropic glutamate receptors, which act via phospholipase C to stimulate IP3-mediated release of Ca2+ from intracellular vesicles3.HOMER proteins have also been implicated in axon guidance during brain development4.HOMER3 can regulate transcription and play a very important role in the differentiation and development for some tissues such as muscle and the nervous system. Studies show that HOMER3 abnormal expression plays a role in acute myeloid leukemia (AML). Heterologous expression of HOMER3 in K562 cells inhibits proliferation, influences the cell cycle profile, affects apoptosis and also promotes cell differentiation5.

Gene ID: 29548

UniProt: Q9Z2X5