MCT3 antibody (C-Term, Intracellular)

Catalog No. ABIN7885146

This Rabbit Polyclonal antibody specifically detects MCT3 in WB. It exhibits reactivity toward Rat and Mouse.

Quick Overview for MCT3 antibody (C-Term, Intracellular) (ABIN7885146)

Target: MCT3 (Monocarboxylate Transporter 3 (MCT3))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCT3 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-MCT3 Antibody

Binding Specificity: AA 421-434, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 3

Predicted Reactivity: Mouse - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RSAEGGASDPEDVE, corresponding to amino acid residues 421-434 of rat MCT3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for MCT3

Target: MCT3 (Monocarboxylate Transporter 3 (MCT3))

Alternative Name: Monocarboxylate transporter 3

Background:

Synonyms: Monocarboxylate transporter 3, Monocarboxylic acid transporter 3, SLC16A8

Description: Proton-linked monocarboxylate transporters (MCTs) are a family of highly homologous trans-membrane symporters, involved in the transportation of monocarboxylic acids across the plasma membrane such as: lactate, pyruvate, branched-chain oxoacids derived from leucine, valine, and isoleucine, ketone bodies, acetoacetate, β-hydroxybutyrate and acetate. Thus, MCTs play an important role in metabolic communication among cells and in mammalian metabolism.MCT3 is encoded by the Slc16a8 gene and contains at least nine members in humans1. Structurally, MCTs have 12 transmembrane spanning domains with cytoplasmic NH2- and COOH-terminals. The COOH-terminal regions are not well conserved among the MCTs isoforms while the membrane spanning domains share the greatest sequence identity3,4. Findings suggest that the N-terminal domain is more important for energy coupling, membrane insertion and correct structure maintenance, whereas the C-terminal domain is more important for the determination of substrate specificity1.MCT3 is expressed in the retinal pigment epithelium, preferentially expressed in the basolateral membrane in the adult and in choroid plexus epithelium where it plays a role in regulating pH and lactate concentrations in the outer retina. Reduced levels of MCT3 in response to trauma or disease could contribute to pathologic changes in the retina2,3. MCT3 can form a heteromeric complex with the accessory protein CD147, an immunoglobulin superfamily protein.

Gene ID: 65200

UniProt: O70461