NEO1 antibody (Extracellular, N-Term)

Catalog No. ABIN7885155

This Rabbit Polyclonal antibody specifically detects NEO1 in WB, IHC, FACS and LCI. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NEO1 antibody (Extracellular, N-Term) (ABIN7885155)

Target: NEO1 (Neogenin 1 (NEO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-NEO1 Antibody

Binding Specificity: AA 701-715, Extracellular, N-Term

Purpose: A Rabbit Polyclonal antibody to Neogenin (extracellular)

Predicted Reactivity: Human,rat - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KIRYRKASRKSDVTE, corresponding to amino acid residues 701 - 715 of mouse Neogenin

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235994)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NEO1

Target: NEO1 (Neogenin 1 (NEO1))

Alternative Name: Neogenin 1

Background:

Synonyms: Neogenin 1, NEO1, NGN

Description: Neogenin also known as NEO1 is a member of the immunoglobulin (Ig) superfamily. It has been reported to be involved in diverse physiology and pathology functions, including cell proliferation, differentiation and migration1. Neogenin was originally isolated from embryonic chicken cerebellum and reported to be homologous to the axon guidance receptor deleted in colorectal cancer (DCC)2.Neogenin consists of 4 N-terminal Ig-like domains, followed by 6 fibronectin type III-like domains, a single transmembrane helix, and an intracellular domain. Neogenin is capable to mediate attractive and repulsive axon guidance depending on the ligand identity. Two main ligands of neogenin are Netrin-1 (NET1) and repulsive guidance molecules (RGMs)3.Interaction of Neogenin with RGMs leads to cytoskeleton rearrangements via Rho GTPases, this results in growth cone collapse. In contrast interaction with Netrin-1 triggers attractive growth cone response3. Recently it was shown that Neogenin plays a role in the maintenance of iron homeostasis via interaction with HJV (members of the RGM family)4.

Gene ID: 18007

UniProt: P97798

Pathways: Transition Metal Ion Homeostasis, Regulation of Muscle Cell Differentiation, Tube Formation