Neuroligin 1 antibody (Extracellular, N-Term)

Catalog No. ABIN7885169

The Rabbit Polyclonal anti-Neuroligin 1 antibody (ABIN7885169) specifically detects Neuroligin 1 in WB, IHC, IF, IC and LCI. The antibody is reactive with Rat and Mouse samples.

Quick Overview for Neuroligin 1 antibody (Extracellular, N-Term) (ABIN7885169)

Target: Neuroligin 1 (NLGN1)

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuroligin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Neuroligin 1 Antibody

Binding Specificity: AA 648-661, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Neuroligin 1

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KVPSTDITLRPTRK, corresponding to amino acid residues 648-661 of rat NLGN1

Isotype: IgG

Application Details

Application Notes:

WB: 1:800

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236049)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Neuroligin 1

Target: Neuroligin 1 (NLGN1)

Alternative Name: NLGN1

Background:

Synonyms: NLGN1, NL1

Description: The NLGN1 gene encodes a member of a family of neuronal cell surface proteins. Neuroligins act as ligands for β-neurexins, cell adhesion proteins located presynaptically. Neuroligin and β-neurexin "shake hands," resulting in the connection between the two neurons and the production of a synapse. Neuroligin 1 may be involved in the formation and remodeling of central nervous system synapses1.Proteins of this family may also mediate signaling by recruiting and stabilizing key synaptic components. This is done by interacting with other postsynaptic proteins to localize neurotransmitter receptors and channels in the postsynaptic density as the cell matures1.Mutations in the neuroligin genes are implicated in autism and other cognitive disorders2.Neuroligins have been found to play a role in angiogenesis, specifically in human pheripheral tissues3.Neuroligins bind with the help of Ca2+ to the α-neurexin LNS (laminin, neurexin and sex hormone-binding globulin-like folding units) domains and to the β-neurexin LNS domain which then establishes a heterophilic trans-synaptic recognition code4. This binding results in a heterotetramer made up of a neuroligin-1 dimer and two neurexin-1 β monomers5.

Gene ID: 116647

UniProt: Q62765

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis