Neuroligin 3 antibody (Extracellular, N-Term)

Catalog No. ABIN7885171

The Rabbit Polyclonal anti-Neuroligin 3 antibody is suitable to detect Neuroligin 3 in samples from Human, Rat and Mouse. It has been validated for WB and IHC.

Quick Overview for Neuroligin 3 antibody (Extracellular, N-Term) (ABIN7885171)

Target: Neuroligin 3 (NLGN3)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuroligin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-Neuroligin 3 Antibody

Binding Specificity: AA 656-671, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Neuroligin 3

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SHITRRPNGKTWSTKR, corresponding to amino acid residues 656-671 of rat NLGN3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236056)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Neuroligin 3

Target: Neuroligin 3 (NLGN3)

Alternative Name: NLGN3

Background:

Synonyms: NLGN3, Gliotactin homolog, HNL3, NL3

Description: Neuroligins (Nlgns) are a family of postsynaptic transmembrane cell adhesion proteins that play a crucial role in excitation and inhibition balance through their regulation of GABAergic and glutamatergic signaling. 5 Neuroligin family members have been identified in humans: NL1, NL2, NL3, NL4X, and NL4Y. NL 1-3 have close homologs in mice. Neurogilins have been identified in rodents, chicken, Drosophila, and C. elegans. Neuroligin structure contains extracellular acetylcholinesterase like domain that lacks esterase activity, a single transmembrane domain, and a short cytoplasmic tail containing a type I PDZ-domain-binding motif that functions in intracellular protein-protein interactions and signaling processes. Neuroligins and neurexins are known to interact with each other1,2.Neuroligin 3 is encoded by the Nlgn3 gene and seems to be ubiquitously expressed in the brain. Despite this fact, Neuroligin 3 functions in synaptic transmission appears to be region specific, affecting the somatosensory cortex and the hippocampus differently2.In humans, a missense mutation in Nlgn3 (Nlgn3R451C) causing reduced synaptic expression of the protein is associated with non-syndromic autism spectrum disorders. In addition, Nlgn3 deletion shows impaired social interaction and vocal communication in rodent models.

Gene ID: 171297

UniProt: Q62889

Pathways: Synaptic Membrane