NMBR antibody (3rd Intracellular Loop)

Catalog No. ABIN7885172

The Rabbit Polyclonal anti-NMBR antibody (ABIN7885172) specifically detects NMBR in WB, IF, IHC and IC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NMBR antibody (3rd Intracellular Loop) (ABIN7885172)

Target: NMBR (Neuromedin B Receptor (NMBR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMBR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC)

Grade: Carrier-free

Product Details anti-NMBR Antibody

Binding Specificity: 3rd Intracellular Loop, AA 241-256

Purpose: A Rabbit Polyclonal Antibody to Bombesin Receptor 1/Neuromedin B Receptor

Predicted Reactivity: Mouse,dog - identical, rat - 15,16 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KSAHNLPGEYNEHTKK, corresponding to amino acid residues 241-256 of human BB1R 

Isotype: IgG

Application Details

Application Notes:

WB: 1:1000

FC: The optimal concentration should be determined by the user

ICC: 1:1000

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236060)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NMBR

Target: NMBR (Neuromedin B Receptor (NMBR))

Alternative Name: BB1R

Background:

Synonyms: BB1R, Neuromedin B receptor, Neuromedin-B-preferring bombesin receptor

Description: Bombesin receptor 1 (BB1) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was initially isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP) and neuromedin B (NMB), 27 and 10 amino acid homologues, respectively.1BB1 is the preferred receptor for NMB (therefore it is also known as NMB receptor) while BB2 is the preferred receptor for GRP.2 BB3 was the third member of the bombesin family of receptors to be cloned based upon its sequence similarity to BB1 and BB2.1 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors, as are its affinities for NMB and GRP. This suggests that the endogenous mammalian ligand for the BB3 receptor remains to be identified.All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB1 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.1BB1 is expressed in the brain where it likely mediates the inhibition of food intake and regulates the stress and fear response. BB1 and its ligand NMB are expressed in the pituitary where they participate in the regulation of thyroid-stimulating hormone (TSH) secretion in both autocrine and paracrine fashions.3 In the periphery, BB1 is expressed in the gastrointestinal and urogenital smooth muscle but its physiological function in these tissues is unclear.Finally, BB1 is over-expressed in several human tumors especially colon and non-small lung cancer where it acts as a growth factor receptor inducing tumor growth. BB1 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4

Gene ID: 4829

UniProt: P28336