NCS1 antibody (Intracellular)

Catalog No. ABIN7885175

The Rabbit Polyclonal anti-NCS1 antibody (ABIN7885175) specifically detects NCS1 in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for NCS1 antibody (Intracellular) (ABIN7885175)

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-NCS1 Antibody

Binding Specificity: AA 40-53, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SGQLDAAGFQKIYK, corresponding to amino acid residues 40-53 of rat NCS1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236071)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NCS1

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Alternative Name: Neuronal calcium sensor 1

Background:

Synonyms: Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP

Description: Neuronal calcium sensor-1 (NCS-1) is a member of the neuronal calcium sensor family of EF-hand Ca2+-binding proteins that triggers different biological processes to regulate signal transduction in neurons and photoreceptor cells. This family of proteins includes 15 members and is primarily expressed in neurons. The protein interacts with the G-protein coupled receptor (GPCR) Dopamine D2 receptor (D2R), and the cognate kinases GRK1 and GRK2. NCS-1 responds to changes in intracellular Ca2+ concentration and is involved in many important physiological functions including the regulation of neurotransmitter release, synaptic plasticity, learning and memory, neurite growth, and neuronal survival1,2.NCS-1 structure is characterized by 4 helix-loop-helix motifs, called EF-hand motifs able to bind Ca2+ ions. These domains are separated by a short loop containing a conserved glycine residue. NCS-1 is targeted to the plasma membrane by an N-terminal myristoylation group through the action of N-miristoyltransferase. Opposite to the EF-motifs is a wide hydrophobic crevice (HC). This area has the ability to interact with various physiological targets2,3.NCS-1 dysfunctions are linked to mental conditions, such as bipolar disorder, schizophrenia and autism2.

Gene ID: 65153

UniProt: P62168