Neuropilin 1 antibody (Extracellular, N-Term)

Catalog No. ABIN7885191

This Rabbit Polyclonal antibody specifically detects Neuropilin 1 in WB, FACS, IF, IC and LCI. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Neuropilin 1 antibody (Extracellular, N-Term) (ABIN7885191)

Target: Neuropilin 1 (NRP1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuropilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Neuropilin 1 Antibody

Binding Specificity: AA 502-514, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Neuropilin-1

Predicted Reactivity: Rat,mouse - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GGKHRENKVFMRK, corresponding to amino acid residues 502-514 of human neuropilin-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: 1:25

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236114)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Neuropilin 1

Target: Neuropilin 1 (NRP1)

Alternative Name: Vascular endothelial cell growth factor 165 receptor

Background:

Synonyms: Vascular endothelial cell growth factor 165 receptor, VEGF165R, CD304

Description: Neuropilins (NRPs) are co-receptors for class 3 semaphorins, polypeptides with key roles in axonal guidance, and for members of the VEGF (vascular endothelial growth factor) family of angiogenic cytokines.Neuropilin 1 (NRP1) is a transmembrane glycoprotein and comprises a large extracellular region containing multiple domains, a single transmembrane domain and a small cytoplasmic domain, also with a number of protein interacting domains.Class 3 semaphorins bind NRP1 (and NRP2) with different, and partially overlapping, specificities and affinities. Plexin A1 and Plexin A2 are the major signaling receptors for class 3 semaphorins, and complex with NRPs and semaphorins to form a holoreceptor essential for mediating effects of semaphorins on growth-cone collapse and axonal guidance in neurogenesis. The major VEGF-A signaling receptor, VEGF receptor 2, forms complexes with NRP1 and is important for optimal VEGFR2 signaling and function in endothelial cells.Targeted disruption of the NRP1 gene has demonstrated an essential dual role of these molecules in neurogenesis and cardiovascular development. NRP1-null mice die with a spectrum of cardiovascular and neuronal defects1.

Gene ID: 8829

UniProt: O14786

Pathways: Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals