PANX1 antibody (Intracellular, N-Term)

Catalog No. ABIN7885220

The Rabbit Polyclonal anti-PANX1 antibody (ABIN7885220) specifically detects PANX1 in WB, IHC and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PANX1 antibody (Intracellular, N-Term) (ABIN7885220)

Target: PANX1 (Pannexin 1 (PANX1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PANX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Carrier-free, KO Validated

Product Details anti-PANX1 Antibody

Binding Specificity: AA 18-31, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Pannexin 1

Predicted Reactivity: Rat,mouse - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KEPTEPKFKGLRLE, corresponding to amino acid residues 18- 31 of human PANX1

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236221)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for PANX1

Target: PANX1 (Pannexin 1 (PANX1))

Alternative Name: PANX1

Background:

Synonyms: PANX1, PX1, MRS1, UNQ2529

Description: Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells1. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2-4.Proteins involved in gap junction formation are composed of four transmembrane domains, two extracellular loops and one intracellular loop and intracellular N- and C-termini5,6. Several consensus cysteine residues are in the extracellular loop and are essential and necessary for intercellular docking of gap junction hemichannels in the opposing cell membrane5-7.Pannexins (Pannexin 1, Pannexin 2 and Pannexin 3) belong to the superfamily of gap junction proteins. Pannexin 1 (PANX1) is ubiquitously expressed, Pannexin 2 (PANX2) is specifically expressed in the human brain and widespread in rodents and Pannexin 3 (PANX3) is also detected in the brain8.The gating properties of Pannexins were studied in Xenopus oocytes and results demonstrate that only PANX1 is able to form homomeric hemichannels, and is also able to form heteromeric hemichannels with PANX2 but not with PANX39,10. Not surprising, PANX1 gating properties depend whether it forms homomeric or heteromeric hemichannels.A number of different stimuli are known to open these channels and include mechanical stress, extracellular ATP, increases in intracellular Ca2+ and inflammation9.Possible roles for Pannexins include paracrine signaling in vascular endothelial cells and taste cell signaling9. Pannexin-1 was shown to interact with NMDA receptors in epilepsy, ischemia, pain and other pathologies10.

Gene ID: 24145

UniProt: Q96RD7