Plexin A2 antibody (Extracellular, N-Term)

Catalog No. ABIN7885233

This Rabbit Polyclonal antibody specifically detects Plexin A2 in WB, IHC, FACS, IF and LCI. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Plexin A2 antibody (Extracellular, N-Term) (ABIN7885233)

Target: Plexin A2 (Plxna2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Plexin A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Plexin A2 Antibody

Binding Specificity: AA 205-218, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Plexin-A2

Predicted Reactivity: Rat,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RDPESSAMLDYELH, corresponding to amino acid residues 205-218 of mouse PLXNA2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236264)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Plexin A2

Target: Plexin A2 (Plxna2)

Alternative Name: PLXNA2

Background:

Synonyms: PLXNA2, PLXN2, Semaphorin receptor OCT

Description: The Plexin gene family is comprised of at least 9 members in 4 subfamilies. Plexins are widely expressed in neurons but have been also detected in epithelial cells1. Plexins closely interact with a large family of proteins called Semaphorins which control a variety of processes in the CNS including cell migration and axonal growth. Plexins and Semaphorins share a common feature of a conserved 500 amino acid "sema" domain2. The ectodomain of PLXNA2 is an N-terminal seven bladed β-propeller sema domain followed by a cysteine rich PSI (plexin-semaphorin-integrin) domain. PLXNA2 has four N-terminal domains and unlike semaphorins the sema domain of unliganded PLXNA2 does not homodimerize readily.Many of the functions of Class 3 semaphorins (Sema3A-Sema3G) are mediated by Plexin-A2 (PLXNA) and other A-plexins. Sema3 signaling depends on semaphorin interacting with one of the two Nrp co-receptors that associate with PLXNA to generate holoreceptors.PLXNA also interacts with semaphorin 6A (Sema6A). Before binding, the Sema6A ectodomain can be found in a 'face-to-face' homodimer arrangement, similar to the arrangement in Sema3, whereas PLXNA is in an unexpected 'head-on' homodimer arrangement. The structure of the Sema6A-PLXNA signaling complex is a 2:2 heterotetramer3.In prostate cancer TMPRSS:ERG gene fusion causes aberrant expression of the transcription factor ERG and the induction of cell migration and invasion. TMPRSS:ERG upregulates PLXNA expression and elevated PLXNA levels were found in prostate cancer cells. Levels of PLXNA were even higher in metastatic prostate cancer than in localized tumors suggesting PLXNA may play a role in the disease's development.

Gene ID: 18845

UniProt: P70207