Plexin A3 antibody (Extracellular, N-Term)

Catalog No. ABIN7885234

The Rabbit Polyclonal anti-Plexin A3 antibody is suitable to detect Plexin A3 in samples from Human, Mouse and Rat. It has been validated for IHC, WB, FACS, IF and LCI.

Quick Overview for Plexin A3 antibody (Extracellular, N-Term) (ABIN7885234)

Target: Plexin A3 (PLXNA3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Plexin A3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Plexin A3 Antibody

Binding Specificity: AA 440-453, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Plexin-A3

Predicted Reactivity: Rat - identical, human - 12,14 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KVRVDGSQDAQLYE, corresponding to amino acid residues 440-453 of mouse PLXNA3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236268)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Plexin A3

Target: Plexin A3 (PLXNA3)

Alternative Name: PLXNA3

Background:

Synonyms: PLXNA3, PLXN3, Semaphorin receptor SEX

Description: Plexins are type 1 transmembrane (TM) receptors acting as key regulatory proteins in a wide variety of developmental, regenerative and pathological processes. They are involved in guidance and motility of vascular, lymphatic vessel, and neuron growth, and in cancer metastasis.Plexin family includes 9 proteins: Plexin-A1-4, -B1-3, -C1 and -D1. Class A Plexins interact with the secreted class 3 Semaphorins, and require Neuropilins (nrps) as co-receptors.Plexin protein signaling results in cytosolic GTPase-activating protein activity including direct interactions with Rho GTPases and transient/catalytic interactions with Ras GTPases1,2.Plexin structure consists of an extracellular semaphorin domain, three Plexin-Semaphorin-Integrin (PSI) domains, and three immunoglobulin, Plexin, and transcription factor (IPT) domains. In addition, a single-spanning transmembrane domain, and a cytosolic region (CYTO) homologous with Ras GTPase-activating proteins (GAPs)2. The transmembrane region of Plexin-A3 is characterized by poly-glycine usually used for close helix-helix packing and aromatic residues that distort helix conformation and association. Plexin-A family members only have 22 residues spanning the membrane1.Mutations in Plexins have been reported in melanoma, lung, breast, pancreatic, and prostate cancers, strongly suggesting that their signaling may play a role in cancer development. Plexins are widely expressed in the nervous system and in the developing cerebral cortex2.

Gene ID: 18846

UniProt: P70208

Pathways: Regulation of Cell Size