Plexin A4 antibody (Extracellular, N-Term)

Catalog No. ABIN7885235

The Rabbit Polyclonal anti-Plexin A4 antibody (ABIN7885235) specifically detects Plexin A4 in WB, IHC, FACS and LCI. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Plexin A4 antibody (Extracellular, N-Term) (ABIN7885235)

Target: Plexin A4 (PLXNA4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Plexin A4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-Plexin A4 Antibody

Binding Specificity: AA 201-215, Extracellular, N-Term

Purpose: A Rabbit Polyclonal antibody to Plexin-A4 (extracellular)

Predicted Reactivity: Human,Rat - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RKLTKNSEADGMFAY, corresponding to amino acid residues 201 - 215 of mouse Plexin-A4

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Plexin A4

Target: Plexin A4 (PLXNA4)

Alternative Name: PLXNA4

Background:

Synonyms: PLXNA4

Description: The plexin family are transmembrane proteins which are classified into four subfamilies: plexin -A, B, C and D. The plexins serve as a receptor for seamaphorins (Sema), axon guidance molecules involved in cellular processes such as axon pruning and repulsion, regulation of cell migration, vascular remodeling and more1. All family members consist of a Sema domain at the N-terminus responsible for Sema binding. In addition, plexins have PSI and IPT (Integrin, Plexin, and Transcription factor) domains, which connect to the transmembrane (TM) helix. Moreover, intracellular region of all plexins contains a membrane proximal juxtamembrane region (JM), a Rho GTPase-binding domain (RBD), and GTPase Activating Protein (GAP) domain2.Plexin-A4 acts as a receptor for the secreted semaphorins Sema3A and Sema6A in the central nervous system, and mediates axon repulsion and synaptic plasticity. Moreover, genome-wide significant association study (GWAS) on humans, has identified that several single nucleotide polymorphisms (SNPs) in PLXNA4 can increase the risk of Alzheimer's disease (AD) 3,4.In the immune system, Plexin-A4 has been implicated in macrophage Toll-like receptor (TLR)-mediated signaling in sepsis, negative regulation of T-cell mediated immune responses in cancer5.

Gene ID: 243743

UniProt: Q80UG2

Pathways: Regulation of Cell Size