RYR3 antibody (Intracellular, N-Term)

Catalog No. ABIN7885376

The Rabbit Polyclonal anti-RYR3 antibody (ABIN7885376) specifically detects RYR3 in IHC, IF, WB and IC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RYR3 antibody (Intracellular, N-Term) (ABIN7885376)

Target: RYR3 (Ryanodine Receptor 3 (RYR3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RYR3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Immunochromatography (IC)

Grade: Carrier-free

Product Details anti-RYR3 Antibody

Binding Specificity: AA 935-949, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Ryanodine Receptor 3

Predicted Reactivity: Rat,mouse - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human RyR3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236746)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for RYR3

Target: RYR3 (Ryanodine Receptor 3 (RYR3))

Alternative Name: RyR3

Background:

Synonyms: RyR3, Brain-type ryanodine receptor

Description: It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1), the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.RyR3 is the most widely expressed RyR channel which is predominantly expressed in smooth muscle tissues and certain regions of the brain.

Gene ID: 6263

UniProt: Q15413

Pathways: Myometrial Relaxation and Contraction