SLIT1 antibody (Secreted)

Catalog No. ABIN7885401

This Rabbit Polyclonal antibody specifically detects SLIT1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SLIT1 antibody (Secreted) (ABIN7885401)

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-SLIT1 Antibody

Binding Specificity: AA 1170-1184, Secreted

Purpose: A Rabbit Polyclonal antibody to SLIT1

Predicted Reactivity: Human,rat - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RDTYLQFTDLQNWPR, corresponding to amino acid residues 1170 - 1184 of mouse SLIT1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SLIT1

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Alternative Name: Slit Guidance Ligand 1

Background:

Synonyms: Slit Guidance Ligand 1, Slit Homolog 1 Protein, Multiple EGF-Like Domains Protein 4, MEGF4

Description: Slit homolog 1 protein (SLIT1), also known as multiple EGF-like domains protein 4, and MEGF4, is a secreted protein member of the SLIT family involved in axon guidance and cell migration.SLIT1 is a member of the SLIT family proteins that includes three members: SLIT1, SLIT2 and SLIT3. SLITS are secreted glycoproteins comprised of four stretches of leucine-rich repeat (LRR) domains, nine epidermal growth factor (EGF) repeats, a Laminin-G-like domain, and a C-terminal cysteine knot.1,2SLITS exert their many physiological roles by binding to Roundabout (Robo) receptors family, that includes four members (Robo 1 to Robo 4), which are single transmembrane receptors belonging to the immunoglobulin superfamily.1,2SLITS play a crucial role in axon guidance during the development of the nervous system. Axon guidance is the process by which neurons extend their axons to reach their appropriate targets, such as other neurons, muscles, or glands. This process is essential for the formation of functional neural circuits.1,3In addition, SLIT1 is involved in various aspects of the regulation of neural progenitor cells (NPCs). NPCs are the precursors to neurons and glial cells in the nervous system. They undergo proliferation, migration, and differentiation to generate the diverse cell types found in the mature brain. SLIT1 is involved in several aspects of NPCs functions, including promoting the proliferation of NPCs in several brain regions, guiding NPCs from the sub-ventricular zone towards the cortical plate and supporting the differentiation of NPCs into specific neuronal or glial cell types.3,4Finally, a complex role for SLIT1 in cancer has emerged. SLIT1 has been ascribed a tumor suppressive role in certain cancers such as breast and colorectal, while in others it appears to associate with tumor progression. In particular, in glioma and pancreatic cancer, SLIT1 expression has been correlated with enhanced metastasis formation and poor prognosis. 2,5,6

Gene ID: 20562

UniProt: Q80TR4

Pathways: Regulation of Cell Size