Sortilin 1 antibody (Extracellular Domain)

Catalog No. ABIN7885532

The Rabbit Polyclonal anti-Sortilin 1 antibody (ABIN7885532) specifically detects Sortilin 1 in WB, IHC, FACS, IF and LCI. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Sortilin 1 antibody (Extracellular Domain) (ABIN7885532)

Target: Sortilin 1 (SORT1)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sortilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Grade: Carrier-free, KO Validated

Product Details anti-Sortilin 1 Antibody

Binding Specificity: AA 320-335, Extracellular Domain

Purpose: A Rabbit Polyclonal Antibody to Sortilin

Predicted Reactivity: Mouse,rat - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237255)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Sortilin 1

Target: Sortilin 1 (SORT1)

Alternative Name: SORT1

Background:

Synonyms: SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95

Description: Sortilin is a member of the Vps10p family named for a yeast gene that is involved in trafficking between the trans-golgi network and the vacuole. In mammals sortilin has been shown to play an important role in golgi to endosome and golgi to lysosome trafficking. Sortilin is also known as neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. In addition, sortilin has lately been identified as a co-receptor of p75NTR for the binding of the proneurotrophins proNGF and proBDNF. Moreover, sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death.Sortilin is a type I membrane protein with a large extracellular domain that includes 8 bacterial neuraminidase repeats (BNRs), a single transmembrane region and a short cytoplasmic tail. The protein is expressed in several tissues, most notably in the brain, spinal cord, skeletal muscle and adipocytes.Paradoxically for its role as either a neurotensin receptor or a p75NTR co-receptor for proneurotrophins, about 90 % of sortilin is retained in intracellular compartments with only about 10 % reaching the cell surface. It has been speculated, that changes in the cellular environment may increase cell surface expression of sortilin.

Gene ID: 6272

UniProt: Q99523

Pathways: Neurotrophin Signaling Pathway