S1PR2 antibody (2nd Cytoplasmic Loop)

Catalog No. ABIN7885537

The Rabbit Polyclonal anti-S1PR2 antibody is suitable to detect S1PR2 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for S1PR2 antibody (2nd Cytoplasmic Loop) (ABIN7885537)

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-S1PR2 Antibody

Binding Specificity: 2nd Cytoplasmic Loop, AA 129-146

Purpose: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 2

Predicted Reactivity: Rat - 17,18 amino acid residues identical, human - 16

Purification: Affinity purified on immobilized antigen.

Immunogen: ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse S1PR2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237271)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for S1PR2

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Alternative Name: Sphingosine 1-phosphate receptor 2

Background:

Synonyms: Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2

Description: Lysophospholipids constitute a group of important lipid mediators, lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2.Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG), S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.Sphingosine 1-phosphate receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5.

Gene ID: 14739

UniProt: P52592

Pathways: Synaptic Membrane