Stim2 antibody (C-Term, Intracellular)

Catalog No. ABIN7885544

The Rabbit Polyclonal anti-Stim2 antibody (ABIN7885544) specifically detects Stim2 in WB, IHC, IF and IC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Stim2 antibody (C-Term, Intracellular) (ABIN7885544)

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Stim2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: Carrier-free, KO Validated

Product Details anti-Stim2 Antibody

Binding Specificity: AA 583-597, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to STIM2

Predicted Reactivity: Rat,mouse,bovine - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237296)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Stim2

Target: Stim2 (Stromal Interaction Molecule 2 (Stim2))

Alternative Name: Stromal interaction molecule 2

Background:

Synonyms: Stromal interaction molecule 2

Description: Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research.Recently, several key players of the store-operated complex have been identified: the Orai family consists of three members: Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane.5 The function of STIM2 still remains elusive and several models have been suggested.6-8

Gene ID: 57620

UniProt: Q9P246

Pathways: TCR Signaling, BCR Signaling