SYNDIG1 antibody (Intracellular, N-Term)

Catalog No. ABIN7885546

The Rabbit Polyclonal anti-SYNDIG1 antibody is suitable to detect SYNDIG1 in samples from Mouse, Rat and Human. It has been validated for WB.

Quick Overview for SYNDIG1 antibody (Intracellular, N-Term) (ABIN7885546)

Target: SYNDIG1 (Synapse Differentiation Inducing 1 (SYNDIG1))

Reactivity: Mouse, Rat, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYNDIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Product Details anti-SYNDIG1 Antibody

Binding Specificity: AA 119-132, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to SynDIG1

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RSPTKDSLEYPDGK, corresponding to amino acid residues 119-132 of rat SynDIG1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237303)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SYNDIG1

Target: SYNDIG1 (Synapse Differentiation Inducing 1 (SYNDIG1))

Alternative Name: Synapse differentiation-inducing gene protein 1

Background:

Synonyms: Synapse differentiation-inducing gene protein 1, Transmembrane Protein 90B, TMEM90B, Dispanin subfamily C member 2, DSPC2

Description: SynDIG1 (Synapse differentiation induced gene 1) is a brain-specific, highly conserved type II integral membrane protein detected at excitatory synapses. The protein is responsible for the regulation of excitatory synapse number and strength in hippocampal neurons. The protein plays an important role during synapse development in regulating AMPA receptor (AMPAR) and PSD-95 content at excitatory synapses1,2.SynDIG family includes four members: SynDIG1-4. SynDIG1 structure includes a large intracellular N-terminal region followed by a single transmembrane domain and a second hydrophobic segment that does not span the membrane. The membrane-associated region forms a three-helical bundle with two cysteine residues located at positions 191 and 192 in the juxta-transmembrane region exposed to the cytoplasm1,2.In studies conducted on mice, deletion of endogenous SynDIG1 in the mouse brain results in a significant decrease in excitatory synapse maturation, but not in synapse composition. Thus, SynDIG1 is not required for synaptogenesis in vivo1. Contrarily, overexpression of SynDIG1 in cultures of dissociated rat hippocampal neurons shows a significant increase in excitatory synapse strength and number.

Gene ID: 362235

UniProt: Q58DZ9

Pathways: Synaptic Membrane